9hh1

LysR Type Transcriptional Regulator LsrB from Agrobacterium tumefaciens

spinqualitylabelsall models 9hh1, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9hh1 is a 2 chain structure with sequence from Agrobacterium fabrum str. C58. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A9CI74_AGRFC Publication Abstract from PubMed Pathogenic bacteria often encounter fluctuating reactive oxygen species (ROS) levels, particularly during host infection, necessitating robust redox-sensing mechanisms for survival. The LysR-type transcriptional regulator (LTTR) OxyR is a widely conserved bacterial thiol-based redox sensor. However, members of the Rhizobiales also encode LsrB, a second LTTR with potential redox-sensing function. This study explores the roles of OxyR and LsrB in the plant-pathogen Agrobacterium tumefaciens. Through single and combined deletions, we observed increased H2O2 sensitivity, underscoring their function in oxidative defense. Genome-wide transcriptome profiling under H2O2 exposure revealed that OxyR and LsrB co-regulate key antioxidant genes, including katG, encoding a bifunctional catalase/peroxidase. Agrobacterium tumefaciens LsrB possesses four cysteine residues potentially involved in redox sensing. To elucidate the structural basis for redox-sensing, we applied single-particle cryo-EM (cryogenic electron microscopy) to experimentally confirm an AlphaFold model of LsrB, identifying two proximal cysteine pairs. In vitro thiol-trapping coupled with mass spectrometry confirmed reversible thiol modifications of all four residues, suggesting a functional role in redox regulation. Collectively, these findings reveal that A. tumefaciens employs two cysteine-based redox sensing transcription factors, OxyR and LsrB, to withstand oxidative stress encountered in host and soil environments. Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens.,Schmidt JJ, Brandenburg VB, Elders H, Shahzad S, Schakermann S, Fiedler R, Knoke LR, Pfander Y, Dietze P, Bille H, Gartner B, Albin LJ, Leichert LI, Bandow JE, Hofmann E, Narberhaus F Nucleic Acids Res. 2025 Mar 20;53(6):gkaf267. doi: 10.1093/nar/gkaf267. PMID:40193708 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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