9hkf

X-Ray crystal structure of a photoswitchable HaloTag bound to JF635

spinqualitylabelsall models 9hkf, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9hkf is a 2 chain structure with sequence from Rhodococcus rhodochrous. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: CL, FMN, GOL, PUJ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A453KFI0_AEGTS DHAA_RHORH Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Expresses halogenase activity against 1-chloroalkanes of chain length C3 to C10, and also shows a very weak activity with 1,2-dichloroethane. Publication Abstract from PubMed Photosensitive fluorophores, whose emission can be controlled using light, are essential for advanced biological imaging, enabling precise spatiotemporal tracking of molecular features and facilitating super-resolution microscopy techniques. Although irreversibly photoactivatable fluorophores are well established, reversible reporters that can be reactivated multiple times remain scarce, and only a few have been applied in living cells using generalizable protein labeling methods. To address these limitations, we introduce chemigenetic photoswitchable fluorophores, leveraging the self-labeling HaloTag protein with fluorogenic rhodamine dye ligands. By incorporating a light-responsive protein domain into HaloTag, we engineer a tunable, photoswitchable HaloTag (psHaloTag), which can reversibly modulate the fluorescence of a bound dye-ligand via a light-induced conformational change. Our best performing psHaloTag variants show excellent performance in living cells, with large, reversible, deep-red fluorescence turn-on upon 450 nm illumination across various biomolecular targets and SMLM compatibility. Together, this work establishes the chemigenetic approach as a versatile platform for the design of photoswitchable reporters, tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging. A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.,Walterspiel F, Ugarte-Uribe B, Weidenhausen J, Vincent M, Narayanasamy KK, Dimitriadi A, Khan AUM, Fritsch M, Muller CW, Zimmermann T, Deo C Angew Chem Int Ed Engl. 2025 Oct 23:e202424955. doi: 10.1002/anie.202424955. PMID:41131894 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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