9hnf

Beta-keto acid cleavage enzyme from Paracoccus denitrificans with bound acetoacetate and acetyl-CoA

spinqualitylabelsall models 9hnf, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9hnf is a 2 chain structure with sequence from Paracoccus denitrificans PD1222. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: AAE, ACO, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A1B802_PARDP Publication Abstract from PubMed The direct reduction of CO(2) into one-carbon molecules is key to highly efficient biological CO(2)-fixation. However, this strategy is currently restricted to anaerobic organisms and low redox potentials. In this study, we introduce the CORE cycle, a synthetic metabolic pathway that converts CO(2) to formate at aerobic conditions and ambient CO(2) levels, using only NADPH as a reductant. Combining theoretical pathway design and analysis, enzyme bioprospecting and high-throughput screening, modular assembly and adaptive laboratory evolution, we realize the CORE cycle in vivo and demonstrate that the cycle supports growth of E. coli by supplementing C1-metabolism and serine biosynthesis from CO(2). We further analyze the theoretical potential of the CORE cycle as a new entry-point for carbon in photorespiration and autotrophy. Overall, our work expands the solution space for biological carbon reduction, offering a promising approach to enhance CO(2) fixation processes such as photosynthesis, and opening avenues for synthetic autotrophy. Design and implementation of aerobic and ambient CO(2)-reduction as an entry-point for enhanced carbon fixation.,Satanowski A, Marchal DG, Perret A, Petit JL, Bouzon M, Doring V, Dubois I, He H, Smith EN, Pellouin V, Petri HM, Rainaldi V, Nattermann M, Burgener S, Paczia N, Zarzycki J, Heinemann M, Bar-Even A, Erb TJ Nat Commun. 2025 Apr 1;16(1):3134. doi: 10.1038/s41467-025-57549-4. PMID:40169551 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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