9i8v

Cryo-EM structure of the Danio rerio tRNA ligase complex

spinqualitylabelsall models 9i8v, resolution 3.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9i8v is a 5 chain structure with sequence from Danio rerio. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.33Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F1R2L8_DANRE RNA helicase.[RuleBase:RU365068] Publication Abstract from PubMed In archaea and metazoa, tRNA exons are ligated by the RNA ligases RtcB and RTCB, respectively. The metazoan RTCB forms a stable complex with four additional subunits, DDX1, FAM98B, CGI99 and ASHWIN. The role and assembly of these four components remain elusive. Furthermore, we lack structural information of how RNA substrates are recognized by 3'-5' tRNA ligases. Here, we use thiol-based chemical cross-linking to confirm the involvement of specific residues of RtcB in RNA binding and we present a cryo-electron microscopy structure of the purified five-subunit D. rerio tRNA ligase complex. The structure implies that the DDX1 helicase module is mobile and can modulate the activity of RTCB. Taken together, the presented results enhance our mechanistic understanding of RNA binding by 3'-5' tRNA splicing ligases and architecture of the metazoan tRNA ligase complex. Structural and biochemical characterization of the 3'-5' tRNA splicing ligases.,Chamera S, Zajko W, Czarnocki-Cieciura M, Jaciuk M, Koziej L, Nowak J, Wycisk K, Sroka M, Chramiec-Glabik A, Smietanski M, Golebiowski F, Warminski M, Jemielity J, Glatt S, Nowotny M J Biol Chem. 2025 Apr 10:108506. doi: 10.1016/j.jbc.2025.108506. PMID:40220997 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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