9im9

CTB10-M4-(S)-1d complex

spinqualitylabelsall models 9im9, resolution 1.81Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9im9 is a 2 chain structure with sequence from Cercospora sp. JNU001. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.81Å Ligands: A1D9Q, GOL, PBF, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A977K7H6_9PEZI Publication Abstract from PubMed The creation of enzymes with abiological abilities offers exciting opportunities to access new-to-nature biocatalysis beyond that found in nature. Here, we repurpose a novel protein scaffold, CTB10, as an artificial photoenzyme through genetic code expansion. It enables catalytic deracemization of cyclopropane, a process that remains inaccessible to traditional biocatalysis due to its thermodynamically unfavorable nature. Following structural optimization through directed evolution, a broad substrate scope with high enantioselectivities is achieved. Furthermore, the crystal structure of the CTB10-based photoenzyme-substrate complex well demonstrates how the catalytic chiral cavity is sculpted to promote efficient and selective light-enabled deracemization. Therefore, this study unlocks the potential for achieving challenging deracemization through biocatalysis. Light-Driven Deracemization by a Designed Photoenzyme.,Li M, Zhang Y, Fu K, Deng Z, Yuan Z, Luo Z, Rao Y J Am Chem Soc. 2025 Apr 23;147(16):13190-13199. doi: 10.1021/jacs.4c16521. Epub , 2025 Apr 12. PMID:40219972 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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