9jff

Crystal structure of L-threonine-O-3-phosphate decarboxylase CobC in complex with reaction intermediate

spinqualitylabelsall models 9jff, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9jff is a 1 chain structure with sequence from Sinorhizobium meliloti. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.15Å Ligands: 33P, CL, GOL, NA, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A499W357_RHIML Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin.[ARBA:ARBA00003444] Publication Abstract from PubMed Vitamin B(12) is involved in many important biochemical reactions for humans, and its deficiency can lead to serious diseases. The industrial production of vitamin B(12) is achieved through microbial fermentation. In this work, we determine the crystal structures of the l-threonine-O-3-phosphate (Thr-P) decarboxylase CobC from Sinorhizobium meliloti (SmCobC), an industrial vitamin B(12)-producing bacterium, in apo form and in complex with a reaction intermediate. Our structures supported the Thr-P decarboxylase activity of SmCobC and revealed that the positively charged substrate-binding pocket between the large and small domains determines its substrate selectivity for Thr-P. Moreover, our results provided evidence for the proposition that the AP-P linker is formed by direct incorporation of AP-P in the biosynthetic pathway of vitamin B(12) in S.meliloti. Crystal structure of l-threonine-O-3-phosphate decarboxylase CobC from Sinorhizobium meliloti involved in vitamin B(12) biosynthesis.,Jiang M, Guo S, Chen X, Wei Q, Wang M Biochem Biophys Res Commun. 2024 Sep 29;734:150767. doi: , 10.1016/j.bbrc.2024.150767. PMID:39366178 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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