9l08

Cyclic Trimer of Helix-Linked Cytochrome c555

spinqualitylabelsall models 9l08, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9l08 is a 2 chain structure with sequence from Aquifex aeolicus VF5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: ACY, GOL, HEC, PEG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Protein-based supramolecules require precise arrangement of building blocks. A regular-triangle trimer (cp-c(555))(3) has been constructed using an alpha-helix-inserted-circular permutant (cp-c(555)) of Aquifex aeolicus cytochrome (cyt) c(555), where the trimers may dissociate to monomers. In this study, we stabilized the regular-triangle structure by constructing a cyclic regular-triangle of three alpha-helix-linked cyt c(555) molecules using sortase-mediated ligation (SML). Comparing SML using sortase A for six cp-c(555) variant trimers, the variant with GGG at the N-terminus and LPETG at the C-terminus reacted most efficiently. OP-(c(555))(3) was designed, in which two cyt c(555) molecules were fused using an alpha-helix, generating a dimer. The cyt c(555) C-terminal region was attached to the N-terminus of the dimer, and the cyt c(555) N-terminal region was attached to the C-terminus of the dimer using the same alpha-helix. OP-(c(555))(3) was expressed in Escherichia coli cells, and the termini were connected by SML, forming a cyclic regular-triangle, CL-(c(555))(3). CL-(c(555))(3) showed higher thermostability than (cp-c(555))(3) and OP-(c(555))(3). CL-(c(555))(3) structural stability was confirmed using high-speed atomic force microscopy. The X-ray crystal structure of CL-(c(555))(3) showed a cyclic structure and a nanoporous supramolecular assembly. These results demonstrate that a nanoporous supramolecular assembly can be constructed by designing a cyclic molecule with a central hole using SML. Construction of a Cyclic Regular-Triangle Trimer of Cytochrome c(555) with a Central Hole Using Sortase A.,Novientri G, Fujiwara K, Mashima T, Matsuura H, Ogata H, Uchihashi T, Fujii S, Sambongi Y, Hirota S Chemistry. 2025 May 14;31(27):e202404736. doi: 10.1002/chem.202404736. Epub 2025 , Apr 16. PMID:40148242 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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