9md9

Photoactivation in Bacteriophytochromes; 3ps TR-SFX structure

spinqualitylabelsall models 9md9, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9md9 is a 2 chain structure with sequence from Stigmatella aurantiaca. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: BEN, BLA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q09E27_STIAD Publication Abstract from PubMed Myxobacteria are non-photosynthetic, soil-dwelling bacteria distinguished by a multicellular stage in their life cycle known as fruiting bodies that are stimulated by light. Myxobacterial phytochromes are candidates for the perception of red-light. The mechanism how light is perceived and converted to a physiological response is unknown. Here, time-resolved serial femtosecond crystallographic (TR-SFX) experiments were conducted on microcrystals of the photosensory core module of the Stigmatella aurantiaca bacteriophytochrome 2 (SaBphP2). Initial events of the Z to E isomerization reaction of the covalently bound, open-chain tetrapyrrole biliverdin (BV) chromophore were determined. At 3 ps after light activation, the BV ring-D assumes a configuration needed for the isomerization. At 100 ps, a mixture of BV in the Z or E configuration is observed in subunit A, while in the other subunit the chromophore remains in the Z configuration. In conjunction with prior results, these structures reveal the molecular mechanism of phytochrome activation in the photomorphogenesis of the myxobacteria and provide the molecular foundation for physiological responses to red light in other bacteria. Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.,Malla TN, Aldama L, Leon V, Feliz D, Hu H, Thomas I, Cellini A, Wahlgren WY, Nimmrich A, Botha S, Sierra R, Hunter MS, Poitevin F, Lisova S, Batyuk A, Gate G, Jernigan R, Kupitz CJ, Maj P, Meszaros P, Kurttila M, Monrroy L, Luo F, Owada S, Kang J, Slavov C, Maj M, Gautier C, Kashipathy M, Tolstikova A, Mariani V, Barty A, Moss F, Schwander P, Liu H, Boutet S, Fromme P, Takala H, Ihalainen JA, Weierstall U, Westenhoff S, Stojkovic EA, Schmidt M Commun Chem. 2025 Jun 12;8(1):183. doi: 10.1038/s42004-025-01578-z. PMID:40506475 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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