9nds

Miniaturized HLA A*02-TAX bound to A6c134 TCR

spinqualitylabelsall models 9nds, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9nds is a 8 chain structure with sequence from Homo sapiens, Human T-cell leukemia virus type I and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.4Å Ligands: NAG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9TPS0_HUMAN Publication Abstract from PubMed The precise recognition of specific peptide-major histocompatibility complex (pMHC) complexes by T cell receptors (TCRs) plays a key role in infectious disease, cancer, and autoimmunity. A critical step in many immunobiological studies is the identification of T cells expressing TCRs specific to a given pMHC antigen. However, the intrinsic instability of empty class-I MHCs limits their soluble expression in Escherichia coli and makes it very difficult to characterize even a small fraction of possible pMHC/TCR interactions. To overcome this limitation, we designed small proteins which buttress the peptide binding groove of class I MHCs, replacing beta2-microglobulin (beta2m) and the heavy chain alpha3 domain, and enable soluble and partially soluble expression in E. coli of H-2D(b) and A*02:01, respectively. We demonstrate that these soluble, monomeric, antigen-receptive, truncated (SMART) MHCs retain both peptide- and TCR-binding specificity and that peptide-bound structures of both allomorphs are similar to their full-length, native counterparts. With extension to the majority of HLA alleles, SMART MHCs should be broadly useful for probing the T cell repertoire in approaches ranging from yeast display to T cell staining. Design of solubly expressed miniaturized SMART MHCs.,White WL, Bai H, Kim CJ, Jude KM, Sun R, Guerrero L, Han X, Chen X, Chaudhuri A, Bonzanini JE, Sun Y, Onwuka AE, Wang N, Wang C, Nygren PA, Li X, Goreshnik I, Allen A, Levine PM, Kueh HY, Jewett MC, Sgourakis NG, Achour A, Garcia KC, Baker D Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2505932123. doi: , 10.1073/pnas.2505932123. Epub 2026 Jan 2. PMID:41481462 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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