9od4

Solution structure of the Ebola Transcriptional Regulatory Signal - configuration B

spinqualitylabelsall models 9od4 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9od4 is a 1 chain structure with sequence from Ebola virus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 10 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The transcription of ebolavirus (EBOV) genomes is a major checkpoint in the viral life cycle. Altering the transcription regulatory signal (TRS), an RNA hairpin that forms in the 3' leader sequence of EBOV, affects transcription in a VP30-dependent manner. How this mechanistically occurs is currently unknown. Here, we find that the TRS primarily equilibrates between two structures that differ in their loops. Mutational studies demonstrate that the entire hairpin structure is important in directing both VP30 binding and regulating EBOV transcriptional activity, with the loop being the principal structural element. In doing so, we highlight that the TRS structure is a critical determinant of EBOV transcription. The Ebolavirus Transcription Regulatory Signal Equilibrates Between Two Structural Conformations to Affect VP30 Binding.,Edwards JM, Kachale A, McCleery SR, Law GA, Patel V, D'Souza VM, Pham VV J Mol Biol. 2025 Sep 25;437(24):169464. doi: 10.1016/j.jmb.2025.169464. PMID:41015113 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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