9oyc

Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped DNA in state 1 conformer 2 with flexibly bound DNA at the shoulder

spinqualitylabelsall models 9oyc, resolution 2.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9oyc is a 11 chain structure with sequence from DNA molecule and Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.71Å Ligands: AGS, MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed DNA sliding clamps are essential for processive DNA synthesis in all domains of life and are loaded by ATP-dependent clamp loaders that recognize recessed 3' ends. How clamp loaders function at nicks and small ssDNA gaps-common intermediates during DNA repair-remains incompletely understood. Here, we show that the bacterial Escherichia coli DnaX clamp loader employs a fundamentally different mechanism from its eukaryotic counterpart. Whereas eukaryotic RFC unwinds DNA at the recessed 3' end and stabilizes the 5'-dsDNA at a dedicated shoulder site, the bacterial DnaX-complex neither unwinds DNA nor stably binds the 5'-dsDNA in vitro. Instead, cryo-EM structures reveal that the beta-clamp itself contains a conserved external DNA-binding site that enables sharp bending of gapped DNA by ~150 degrees , promoting insertion of the 3'-dsDNA into the clamp. This DNA-bending mechanism allows efficient beta-clamp loading at nicks and small gaps and reveals a distinct bacterial strategy for clamp loading. Because small DNA gaps are frequently associated with DNA damage, clamps loaded at these sites are likely important for DNA repair. The E. coli DnaX clamp loader sharply bends DNA to load beta-clamp at nicks and small gaps.,Zheng F, Yao NY, Georgescu RE, Lyu M, O'Donnell ME, Li H bioRxiv [Preprint]. 2026 Jan 20:2026.01.17.700081. doi: , 10.64898/2026.01.17.700081. PMID:41648351 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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