9ozy

Gradient equilibration of hexagonal thermolysin to low salt over 15 minutes

spinqualitylabelsall models 9ozy, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9ozy is a 1 chain structure with sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.75Å Ligands: CA, DMS, LYS, VAL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function THER_GEOSE Extracellular zinc metalloprotease. Publication Abstract from PubMed We describe a device and a method for changing the ambient solution of a macromolecular crystal. The approach is gentle, automated, inexpensive and open source. Examples are given of the equilibration of three different crystals to new solutions with exchange times ranging from 5 to 180 min. In each case direct transfer of the crystal to the new solution causes cracking, which is eliminated with gradient equilibration using the described device. Crystals equilibrated with the device produce high-quality diffraction that yields refined structures comparable to those determined previously. The device offers a more systematic and labor-saving workflow than current practice both for performing diffraction analysis of macromolecular crystals and for investigating the response of macromolecular crystals to changes in solution composition. Automated gradient equilibration of macromolecular crystals to new solution conditions.,Juers DH, Quire J, Stothers S Acta Crystallogr F Struct Biol Commun. 2025 Nov 1. doi: , 10.1107/S2053230X25008398. PMID:41042616 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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