9pz6
Anti-NANP Fab with G112T light chain mutation
Structural highlights
Publication Abstract from PubMedMonoclonal antibodies with lambda (lambda) light chains are less commonly used in therapeutics due to their lower biophysical stability compared to kappa (kappa) variants. Here, we identify a conserved glycine residue (Gly111) in the lambda light chain hinge as a driver of large-scale Fab elbow-angle transitions. Using microsecond-scale molecular dynamics simulations of the EBV-neutralizing Fab AMMO1, we show that substituting Gly111 with threonine (G111T) increases the free energy barrier between conformational states, effectively arresting these transitions. Structural and biophysical analyses-including crystallography, differential scanning fluorimetry, and surface plasmon resonance-confirm that the mutation maintains Fab architecture and antigen binding while increasing thermal stability by up to 2.5 degrees C. The same mutation applied to a second lambda-Fab yielded similar stabilization, and simulations of three clinical lambda-Fabs revealed consistent reductions in elbow-angle flexibility. These results demonstrate a generalizable, single-residue engineering strategy to enhance the stability of lambda-based Fabs without compromising function, with direct implications for therapeutic antibody development and manufacturability. Single-residue engineering of lambda (lambda) antibody light chains reduces conformational flexibility and enhances thermal stability.,Jewel Y, Young T, Park M, Ly K, Gonzalez A, Mallett TC, Williams JC Comput Struct Biotechnol J. 2025 Oct 24;27:4730-4739. doi: , 10.1016/j.csbj.2025.10.045. eCollection 2025. PMID:41245890[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||||
