9r45

Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Xylose isomerase (apo state)

spinqualitylabelsall models 9r45, resolution 1.79Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9r45 is a 1 chain structure with sequence from Streptomyces pseudogriseolus group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.79Å Ligands: MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function XYLA_STRRU Involved in D-xylose catabolism. Publication Abstract from PubMed We previously introduced the spitrobot, a protein crystal plunging system that enables reaction quenching via cryo-trapping with a time resolution in the millisecond range. Here we present the next generation, spitrobot-2, as an integrated benchtop device. User-friendliness has been improved by semi-automatic sample exchange. Moreover, a fully automated shutter shields the liquid nitrogen from the humidified environment, improving sample integrity. Most importantly, the cryo-trapping delay time has been reduced to 23 ms, making spitrobot-2 twice as fast as the previous generation. This further expands the number of target systems that can be addressed by cryo-trapping time-resolved crystallography. Using 12 crystal structures of three independent model systems, we demonstrate successful cryo-trapping via observation of conformational changes and ligand binding within 25 ms. These improvements increase the convenient access to cryo-trapping, time-resolved X-ray crystallography empowering the MX community with efficient tools to advance research in structural biology. Spitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms.,Spiliopoulou M, Hatton CE, Kollewe M, Leimkohl JP, Schikora H, Tellkamp F, Mehrabi P, Schulz EC Commun Chem. 2025 Nov 20;8(1):363. doi: 10.1038/s42004-025-01784-9. PMID:41266557 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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