9raj

Class A CTX-M-14 E166A mutant in complex with Dicloxacillin at room temperature

spinqualitylabelsall models 9raj, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9raj is a 1 chain structure with sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.7Å Ligands: DXU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H3H219_KLEPH Publication Abstract from PubMed The predominant resistance mechanism observed in Gram-negative bacteria involves the production of beta-lactamases, which catalyse the hydrolysis of beta-lactam antibiotics, thereby rendering them ineffective. Although Isoxazolyl Penicillins have been available since the 1970s, there are currently no structures in complex with class-A beta-lactamases available. Here we have analysed the structure of the clinically relevant beta-lactamase CTX-M-14 from Klebsiella pneumoniae near physiological temperatures, via serial synchrotron crystallography. We demonstrate the acyl-enzyme intermediates of the catalytically impaired CTX-M-14 mutant E166A in complex with three Isoxazolyl-Penicillins: Oxacillin, Cloxacillin and Dicloxacillin. Structural comparisons of CTX-M-Penicillin complexes show that, while conserved active-site interactions are maintained, each Isoxazolyl-Penicillin adopts a distinct conformation. While the three derivatives differ only by one and two chlorine atoms, respectively, their conformational heterogeneity appears to be increased by chlorination of the phenyl ring. Binding mode of Isoxazolyl Penicillins to a Class-A beta-lactamase at ambient conditions.,Gore G, Prester A, von Stetten D, Bartels K, Schulz EC Commun Chem. 2025 Dec 1;8(1):387. doi: 10.1038/s42004-025-01801-x. PMID:41326695 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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