9rjs

Structure of the Bacteriophage PhiKZ non-virion RNA Polymerase bound to an analogue of its promoter

spinqualitylabelsall models 9rjs, resolution 2.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9rjs is a 7 chain structure with sequence from Pseudomonas virus phiKZ. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.59Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8SD88_BPDPK Publication Abstract from PubMed Bacteriophage PhiKZ (PhiKZ) is the founding member of a family of giant bacterial viruses. It has potential as a therapeutic as its host, Pseudomonas aeruginosa, kills tens of thousands of people worldwide each year. PhiKZ infection is independent of the host transcriptional apparatus; the virus forms a "nucleus", producing a proteinaceous barrier around the PhiKZ genome that excludes the host immune systems. It expresses its own non-canonical multi-subunit non-virion RNA polymerase (nvRNAP), which is imported into its "nucleus" to transcribe viral genes. The PhiKZ nvRNAP is formed by four polypeptides representing homologues of the eubacterial beta/beta' subunits, and a fifth that is likely to have evolved from an ancestral homologue to sigma-factor. We have resolved the structure of the PhiKZ nvRNAP initiating transcription from its cognate promoter, p119L, including previously disordered regions. Our results shed light on the similarities and differences between PhiKZ nvRNAP mechanisms of transcription and those of canonical eubacterial RNAPs and the related non-canonical nvRNAP of bacteriophage AR9. Structure of the Bacteriophage PhiKZ Non-virion RNA Polymerase Transcribing from its Promoter p119L.,de Martin Garrido N, Chen CS, Ramlaul K, Aylett CHS, Yakunina M J Mol Biol. 2024 Jul 17;436(18):168713. doi: 10.1016/j.jmb.2024.168713. PMID:39029888 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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