9s2a
X-ray structure of lysozyme obtained upon reaction with the dioxidovanadium(V) complex with thiophene-2-carboxylic acid (3-ethoxy-2-hydroxybenzylidene)hydrazide
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedThree new water-stable aqueous dioxidovanadium(V) complexes, [(V(V)O(2)L(1-3))M(H(2)O)(n)] (1-3), incorporating hydrazone ligands with different alkali metals (Na(+)/K(+)) as counterions were synthesized and characterized by various physicochemical approaches, including single-crystal X-ray diffraction (SCXRD). Time-dependent spectroscopic/spectrometric techniques were used to determine their aqueous-phase stabilities. Blood compatibility studies were employed to investigate their efficacy and stability with human red blood cells. Lipophilicity and calf thymus (CT)-DNA interaction of 1-3 were investigated using conventional techniques. High-resolution molecular structures of the adduct formed between 1 and hen egg white lysozyme (HEWL) were determined by SCXRD. The structural analysis reveals that the compound self-assembles within protein crystals, forming a dimeric structure that non-covalently interacts with the protein surface. The binding of 1 to HEWL was also evaluated through different spectroscopic methods. Fluorescence data indicate that 1 can also bind the physiologically relevant protein human serum albumin at pH 7.4. Furthermore, the cytotoxicity of 1-3 was evaluated against the lung (A549) and human breast adenocarcinoma (MCF-7) cancer cell lines, as well as an human embryonic kidney cell line (HEK-293) noncancerous cell line. 1 (IC(50) value of 9.2 +/- 0.1 muM) is more effective than the other two complexes. It induces cell death via apoptosis. Binding of Aqueous-Stable, Lipophilic, Hemocompatible Anticancer V(V)O(2) Metallodrugs with Biological Molecules: X-ray Structures of the Adduct of the V(V)-hydrazonato Complex with Hen Egg White Lysozyme.,Mohapatra D, Pattanayak PD, Kaminsky W, Paolillo M, Tito G, Ferraro G, Merlino A, Dinda R Inorg Chem. 2026 Mar 30;65(12):6412-6433. doi: 10.1021/acs.inorgchem.5c05201. , Epub 2026 Mar 14. PMID:41830615[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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