9s39

Serial crystallography structure of a photosynthetic reaction center using a goniometer-compatible 96-well chip-based platform

spinqualitylabelsall models 9s39, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9s39 is a 5 chain structure with sequence from Blastochloris viridis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.3Å Ligands: BPB, FE2, FME, HEC, MQ7, NS5, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CYCR_BLAVI The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor. Publication Abstract from PubMed Fixed-target platforms provide convenient support for microcrystals during serial X-ray crystallography studies using synchrotron radiation. Here, we describe a simple user-friendly 3D-printed support where the crystals are sandwiched between two layers of thin X-ray-transparent membrane resulting in very low scattering background. The platform is compatible with magnetic mounting onto the standard goniometer of macromolecular crystallography beamlines. Our design utilizes a 96-well frame that facilitates hanging-drop experiments directly on the membrane using conventional crystallization plates, thereby eliminating multiple pipetting and crystal handling steps. Crystals can be enclosed in a sandwich and packed into 'cassettes', preventing the risk of the sample drying out during room-temperature transportation to synchrotron sources. The versatility of the platform is demonstrated by five structures solved using different crystallization and data-collection strategies. Lysozyme single-crystal rotational crystallography at room temperature is shown, as well as microcrystal serial data collection under cryogenic conditions. On-chip microcrystallization is illustrated by use of a photosynthetic reaction center as an example. Finally, serial crystallography data collection at room temperature from microcrystals of the membrane protein cytochrome c oxidase crystallized in lipidic cubic phase is presented. A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons.,Ghosh S, Banacore A, Norder P, Bjelcic M, Kabbinale A, Nileshwar P, Wehlander G, de Sanctis D, Basu S, Orlans J, Vallejos A, Chavas LMG, Neutze R, Branden G J Appl Crystallogr. 2026 Feb 17;59(Pt 2):303-315. doi: 10.1107/S1600576725011513. , eCollection 2026 Apr 1. PMID:41959872 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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