9s7c

Structure of human mitochondrial COX1-translating ribosome nascent chain complex with tRNAs in initial hybrid state (H1)

spinqualitylabelsall models 9s7c, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9s7c is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.2Å Ligands: 1MA, 2MG, 5F0, 5MC, 5MU, ACE, ATP, B8T, FES, GDP, K, MA6, MG, NAD, OMG, OMU, PSU, VAL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RM02_HUMAN Publication Abstract from PubMed The human mitochondrial genome encodes 13 subunits of the oxidative phosphorylation system essential for energy metabolism to drive cellular activities. Translation of 11 mRNAs by membrane-bound ribosomes is coupled to insertion of the nascent polypeptides into the inner membrane aided by the OXA1L insertase. To this end, the mechanism of membrane insertion of nascent polypeptides and the functional link to the translation process are not sufficiently understood. Here, we applied ribosome profiling to assess translation dynamics in combination with cryo-electron microscopy analysis of a COX1 ribosome-nascent chain complex to visualize cotranslational folding of the nascent chain. We find that the membrane topology of the translation product impacts translation speed and that positioning of amphipathic helices in the ribosome vestibule induces structural changes, correlating with translation pausing events. Thus, our findings reveal a link between translation process and folding and membrane insertion of nascent polypeptides at the inner mitochondrial membrane. Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed.,Schondorf T, Petrychenko V, Kotan I, Dahal D, Napieraj N, Cruz-Zaragoza LD, Wang C, Urbach O, Gall T, Dennerlein S, Kramer G, Fischer N, Rehling P Nat Struct Mol Biol. 2026 May;33(5):853-867. doi: 10.1038/s41594-026-01803-w. , Epub 2026 May 7. PMID:42098403 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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