| Structural highlights
9sau is a 4 chain structure with sequence from Synechocystis sp. PCC 6803. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | Electron Microscopy, Resolution 3.6Å |
| Ligands: | 5X6, A1JR2, CLA, GRG, LMN, MGE |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
HLID_SYNY3 Involved in photosystem II (PSII) assembly and/or repair under high light stress (PubMed:24681620). Required for binding of chlorophyll and carotenoids by the Ycf39-Hlip complex. The Ycf39-Hlip complex binds D1 at an early stage of PSII assembly along with Ycf48, ribosomes and ChlG, the last enzyme in chlorophyll biosynthesis; it may be involved in chlorophyll reuse and delivery to D1 in the initial stages of PSII assembly (PubMed:24681620). Binds chlorophyll a and beta-carotenoid in a 3:1 stoichiometry in the presence and absence of Yfc39; in the Ycf39-HliC-HliD complex, HliD binds all the pigment. The Ycf39-Hlip complex efficiently quenches chlorophyll fluorescence, contributing to photoprotection (PubMed:25706339). Deletion of 4 to 5 members of the Hlip family suggests the proteins are involved in regulation of chlorophyll biosynthesis, in stabilization of chlorophyll-binding proteins and/or in reuse of chlorophylls, and may regulate tetrapyrrole biosynthesis (Probable). Might bind chlorophyll and/or carotenoids in association with HliC (called the ScpBE pair) (Probable).[1] [2] [3] [4] The Hlips might regulate tetrapyrrole biosynthesis, maybe at the level of aminolevulinic acid synthesis and probably stabilize PSII assembly intermediates (PubMed:11999371, PubMed:22090028).[5] [6]
References
- ↑ Knoppova J, Sobotka R, Tichy M, Yu J, Konik P, Halada P, Nixon PJ, Komenda J. Discovery of a chlorophyll binding protein complex involved in the early steps of photosystem II assembly in Synechocystis. Plant Cell. 2014 Mar;26(3):1200-12. doi: 10.1105/tpc.114.123919. Epub 2014 Mar , 28. PMID:24681620 doi:https://dx.doi.org/10.1105/tpc.114.123919
- ↑ Staleva H, Komenda J, Shukla MK, Slouf V, Kana R, Polivka T, Sobotka R. Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins. Nat Chem Biol. 2015 Apr;11(4):287-91. doi: 10.1038/nchembio.1755. Epub 2015 Feb , 23. PMID:25706339 doi:https://dx.doi.org/10.1038/nchembio.1755
- ↑ Xu H, Vavilin D, Funk C, Vermaas W. Multiple deletions of small Cab-like proteins in the cyanobacterium Synechocystis sp. PCC 6803: consequences for pigment biosynthesis and accumulation. J Biol Chem. 2004 Jul 2;279(27):27971-9. doi: 10.1074/jbc.M403307200. Epub 2004 , Apr 23. PMID:15107425 doi:https://dx.doi.org/10.1074/jbc.M403307200
- ↑ Storm P, Hernandez-Prieto MA, Eggink LL, Hoober JK, Funk C. The small CAB-like proteins of Synechocystis sp. PCC 6803 bind chlorophyll. In vitro pigment reconstitution studies on one-helix light-harvesting-like proteins. Photosynth Res. 2008 Oct-Dec;98(1-3):479-88. doi: 10.1007/s11120-008-9368-0. Epub , 2008 Oct 3. PMID:18836846 doi:https://dx.doi.org/10.1007/s11120-008-9368-0
- ↑ Xu H, Vavilin D, Funk C, Vermaas W. Small Cab-like proteins regulating tetrapyrrole biosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol. 2002 May;49(2):149-60. doi: 10.1023/a:1014900806905. PMID:11999371 doi:https://dx.doi.org/10.1023/a:1014900806905
- ↑ Yao DCI, Brune DC, Vavilin D, Vermaas WFJ. Photosystem II component lifetimes in the cyanobacterium Synechocystis sp. strain PCC 6803: small Cab-like proteins stabilize biosynthesis intermediates and affect early steps in chlorophyll synthesis. The Journal of biological chemistry. 2012 Jan 2. doi: 10.1074/jbc.M111.320994. PMID: 22090028.
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