9t0x

Crystal structure of H416C NikA mutant from Escherichia coli covalently bound to a modified Mn(salen) complex

spinqualitylabelsall models 9t0x, resolution 1.81Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9t0x is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.81Å Ligands: LCY Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NIKA_ECOLI Involved in a nickel transport system, probably represents the nickel binder. Publication Abstract from PubMed Artificial enzymes represent a promising alternative for performing non-natural reactions in biocatalysis. Here, we illustrate the potential of cross-linked enzyme crystals (CLEC) to achieve enantioselective epoxidation through the generation of an artificial enzyme obtained by direct covalent anchoring of a manganese complex as an artificial active site within a protein. Enantiomeric excess (ee) of up to 90% on cis-beta-methylstyrene was measured when the covalent binding yield was maximized, thanks to the remarkable behavior of the crystals. The structure of the modified enzyme, NikA, is provided. This work adds to the growing body of examples highlighting the advantages of CLEC in oxidation catalysis. Covalent Insertion of a Mn(Salen) Type Complex in Cross-Linked Protein Crystals: Design of an Enantioselective Artificial Epoxidase.,Boukhallat M, Benhamed I, Arnone J, Van Baaren S, Rinaldi C, Catty P, Marchi-Delapierre C, Cavazza C, Menage S Chemistry. 2026 May 29:e71159. doi: 10.1002/chem.71159. PMID:42210913 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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