9tb4

SAM-dependent C6-FPP methytransferase from Streptomyces varsoviensis in complex with SAH and FPP

spinqualitylabelsall models 9tb4, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9tb4 is a 12 chain structure with sequence from Streptomyces. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: EDO, FPP, PE8, PEG, PG4, PGE, SAH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Non-canonical methylation events generate terpene structures that evade classical biosynthetic predictions, as exemplified by the proposed C(16) terpene hegelenether. Here, we show that this natural product is misassigned and revise its structure to the dihydroxylated sesquiterpenoid marxdiol. Its absolute configuration and that of its precursor prekantenol pyrophosphate were determined through terpene synthase-mediated incorporation of stereoselectively labeled probes. To explain the initiating C6 methylation, we solved the crystal structure of the methyltransferase C6-FPP-MT with SAH and FPP, revealing a compact aromatic pocket that enforces Si-face methylation and Glu165-mediated deprotonation. These insights define how the active site controls regio- and stereochemistry and provide a structural basis for identifying related methyl-modified terpenes in uncharacterized biosynthetic pathways. Structural Revision of the C(16) Sesquiterpene Hegelenether and the Mechanism of C6-Methylation in Terpene Biosynthesis.,Li H, Schnakenburg G, Groll M, Dickschat JS Angew Chem Int Ed Engl. 2026 Jan 15:e25672. doi: 10.1002/anie.202525672. PMID:41540682 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA