9uah

Crystal structure of the OkaE-W79A mutant with a-ketoglutarate and okaramine A

spinqualitylabelsall models 9uah, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9uah is a 4 chain structure with sequence from Penicillium simplicissimum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: AKG, CO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed alpha-Ketoglutarate-dependent mononuclear non-haem iron (alphaKG-NHFe) enzymes are catalytically versatile, yet OkaE is unique for synthesizing azetidine rings via C-C bond formation. Here, we report the unexpected multifunctionality of OkaE, which catalyzes sequential oxidations. Isotopic labelling studies demonstrate that a second O(2) molecule participates in sequential epoxidation and ring cleavage, incorporating two oxygen atoms within a single catalytic cycle to form the previously unknown structure, neuokaramine IV. Crystal structures of the OkaE*Co(II)*alphaKG*okaramine A complex unveil a unique methionine-pi interaction network that facilitates substrate binding. Mutational and crystallographic analyses suggest this network fine-tunes substrate orientation relative to the metallo-centre, activating distinct reaction pathways at the 3a-OH or C8a positions. QM/MM simulations indicate that dynamic rotation of the Fe(IV)=O species initiates the cycle, enabling reaction bifurcation. This study elucidates the structural and mechanistic basis of OkaE's reactivity, highlighting its potential as a programmable biocatalyst for natural product diversification. Structural and mechanistic insights into azetidine-associated alphaKG-NHFe enzyme OkaE with multifunctional catalysis.,Wang X, Yu J, Liu T, Zhang X, Ju M, Xie Z, Naowarojna N, Ping L, Dong Y, Gong B, Xie Y, Nie Y, Hsiang T, Wu R, Zhang L, Liu P, Zhu G, Yan W, Liu X Nat Commun. 2026 Feb 17. doi: 10.1038/s41467-026-69519-5. PMID:41702921 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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