9uow

Crystal structure of Gossypium hirsutum (Cotton) 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) in open conformation

spinqualitylabelsall models 9uow, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9uow is a 3 chain structure with sequence from Gossypium hirsutum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: EDO, GOL, GPJ, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A7Y7Y2_GOSHI Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.[ARBA:ARBA00002174] Publication Abstract from PubMed Cotton (Gossypium spp.), a major fiber crop, requires effective weed control to maintain optimal productivity. Glyphosate targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme in aromatic amino acid biosynthesis; however, the structural mechanisms underlying its inhibition and resistance in plants remain poorly understood. Here, we present the 2.2 A three-dimensional structure of glyphosate-sensitive GhEPSPS from cotton in its open conformation. A G178A variant showed markedly reduced glyphosate sensitivity, with an IC(5)(0) of 22.27 mM and a dissociation constant of 419 muM compared with 16.4 and 2.7 muM for the wild type. MD simulations revealed that G178A disrupts glyphosate binding, whereas the P183L and TIPS variants remain structurally stable and are unlikely to impair catalytic efficiency. High-throughput virtual screening further identified potential herbicidal chemotypes, and isothermal titration calorimetry confirmed that one of the top candidates, CHEMBL1383715, binds GhEPSPS with a K(d) of 34.5 muM. These findings provide structural insights into glyphosate resistance and highlight CHEMBL1383715 as a promising scaffold for next-generation EPSPS-targeting herbicides. Structural and Biochemical Insights into Cotton 5-Enolpyruvylshikimate-3-Phosphate Synthase: Toward Inhibitor Design and Glyphosate Resistance.,Jangid K, Chaudhary D, Verma S, Das J, Baksi S, Sharma AK, Kumar R, Kumar P J Agric Food Chem. 2025 Dec 23. doi: 10.1021/acs.jafc.5c08286. PMID:41432417 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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