Structural highlights
9vnj is a 2 chain structure with sequence from Acinetobacter sp. Tol 5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 2.6Å |
| Ligands: | C8E, CA, CL, MES, PGE |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
ATAA_ACIS5 Responsible for autoagglutination, and for adhesion to abiotic and biotic surfaces such as polystyrene (PS), type I collagen, polypropylene (PP), polyvinylchloride (PVC), glass and stainless steel (SS). Adhesion is much stronger than that mediated by Yersinia YadA in a comparative assay. Confers autoagglutination and binding to PS, type I collagen, PP, PVC, glass and SS upon expression in Acinetobacter baylyi strain ADP1 (PubMed:23155410). Involved in rapid, irreversible adherence to polyurethane (PubMed:17090933). Forms an unusual biofilm (PubMed:31972092). An extended, surface exposed fiber binds to quartz crystals, PS and glass. It can be removed by washing in distilled water (PubMed:27305955, PubMed:28720107).[1] [2] [3] [4] [5]
References
- ↑ Ishii S, Unno H, Miyata S, Hori K. Effect of cell appendages on the adhesion properties of a highly adhesive bacterium, Acinetobacter sp. Tol 5. Biosci Biotechnol Biochem. 2006 Nov;70(11):2635-40. PMID:17090933 doi:10.1271/bbb.60236
- ↑ Ishikawa M, Nakatani H, Hori K. AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces. PLoS One. 2012;7(11):e48830. PMID:23155410 doi:10.1371/journal.pone.0048830
- ↑ Yoshimoto S, Nakatani H, Iwasaki K, Hori K. An Acinetobacter trimeric autotransporter adhesin reaped from cells exhibits its nonspecific stickiness via a highly stable 3D structure. Sci Rep. 2016 Jun 16;6:28020. PMID:27305955 doi:10.1038/srep28020
- ↑ Yoshimoto S, Ohara Y, Nakatani H, Hori K. Reversible bacterial immobilization based on the salt-dependent adhesion of the bacterionanofiber protein AtaA. Microb Cell Fact. 2017 Jul 18;16(1):123. PMID:28720107 doi:10.1186/s12934-017-0740-7
- ↑ Furuichi Y, Yoshimoto S, Inaba T, Nomura N, Hori K. Process Description of an Unconventional Biofilm Formation by Bacterial Cells Autoagglutinating through Sticky, Long, and Peritrichate Nanofibers. Environ Sci Technol. 2020 Feb 18;54(4):2520-2529. PMID:31972092 doi:10.1021/acs.est.9b06577
