9vy2

Crystal structure of antitoxin VP14460 (PoNi)from Vibrio parahaemolyticus

spinqualitylabelsall models 9vy2, resolution 2.32Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9vy2 is a 2 chain structure with sequence from Vibrio parahaemolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.32Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A024B384_VIBPH Publication Abstract from PubMed Vibrio parahaemolyticus, a halophilic pathogen, contaminates seafood and high-salt foods, posing significant health risks such as gastroenteritis and fatalities. With increasing seafood consumption, developing effective control strategies is imperative. The type VI secretion system, a common molecular weapon in Vibrio that mediates cross-domain interactions, is a contractile nanomachine that deploys antibacterial effectors, each paired with a cognate immunity protein to form effector-immunity (E-I) modules. Here, we present the first structural and functional characterization of this complex. The crystal structure of the VP14460-VP14465 complex unveils the molecular basis for specific E-I recognition, defining a set of critical interfacial residues. Structure-guided mutagenesis confirmed that these residues are essential for complex integrity, their disruption liberates the VP14465 toxin, unleashing potent bactericidal activity. Furthermore, we identified the active sites responsible for the DNase activity of VP14465. Notably, while VP14460 and VP14465 form a stable monomeric complex in the bound state, each isolated component exhibits distinct oligomeric behavior in vitro. This suggests a dynamic assembly-disassembly mechanism that may regulate effector delivery and toxin activation during interbacterial competition. Collectively, our findings provide mechanistic insights into type VI secretion system E-I module architecture and function, and establish a structural framework for the development of novel antibacterial therapeutics targeting DNase family effectors. Structural and mechanistic insights into the VP14460-VP14465 effector-immunity module of the Vibrio parahaemolyticus type VI secretion system.,Zheng Y, Zheng C, Ye Z, Huang L, Lin X, Wu B, Pan Z, Qiu R, Cai J, Xu L, Deng Z, Xu R, Xie X, Xie L, Hu F J Biol Chem. 2026 Jun 15;302(8):113257. doi: 10.1016/j.jbc.2026.113257. PMID:42297241 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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