9w3i

Cryo-EM structure of Oryza sativa phosphate transporter SPDT in apo-state

spinqualitylabelsall models 9w3i, resolution 3.16Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9w3i is a 2 chain structure with sequence from Oryza sativa Indica Group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.16Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1Y0BRS7_ORYSI Publication Abstract from PubMed Phosphorus (P) limitation severely affects crop yields. To address the molecular basis of inorganic phosphate (Pi) specific transport within the sulfate transporter (SULTR) family, we determined the cryo-electron microscopy structures of Oryza sativa SULTR-like phosphorus distribution transporter (OsSPDT), a key Pi transporter for grain allocation, in apo- and Pi-binding states. OsSPDT forms a domain-swapped homodimer with each protomer containing an N-terminal domain (NTD), a transmembrane domain (TMD) divided into core and gate subdomains, and a C-terminal sulfate transporter and antisigma factor (STAS) domain. The structure adopts a cytoplasm-facing conformation with Pi coordinated at the core-gate interface. Key residues, including SPDT-unique Ser(170), mediate Pi specificity within the binding pocket, distinguishing it evolutionarily from sulfate transporters within the SULTR family. Domain-swapping and mutational studies demonstrate functional interdependence of the NTD, TMD, and STAS domains. This work elucidates Pi selectivity in plant SULTR transporters and provides a molecular basis for developing low-phytate rice via OsSPDT gene editing. Molecular mechanism underlying phosphate distribution by SULTR family transporter SPDT in Oryza sativa.,Fang S, Zhao Y, Zhang X, Yu F, Zhang P Sci Adv. 2025 Oct 10;11(41):eady3442. doi: 10.1126/sciadv.ady3442. Epub 2025 Oct , 10. PMID:41071878 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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