9wpn

Crystal structure of the nitrilase superfamily protein CJ1056C from Campylobacter jejuni in space group P212121

spinqualitylabelsall models 9wpn, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9wpn is a 6 chain structure with sequence from Campylobacter jejuni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A5T0F3Q1_CAMJU Publication Abstract from PubMed The nitrilase superfamily comprises enzymes that hydrolyze nonpeptide carbon-nitrogen bonds in nitriles and amides and contribute to diverse cellular processes, such as signaling, metabolism, and detoxification. These enzymes rely on the canonical Cys-Glu-Lys catalytic triad for their nitrilase or amidase activities. We identified a gene product of Campylobacter jejuni (CJ1056C) belonging to the nitrilase superfamily and determined its unique structure by X-ray crystallography in two crystal forms at resolutions of 2.3 A and 2.9 A. CJ1056C folds into a four-layered alphabetabetaalpha structure with a pocket, as observed in typical nitrilase superfamily proteins, such as Nit2 and aliphatic amidase. However, the catalytic triad of the nitrilase superfamily which is generally located at the base of the pocket, is disrupted in CJ1056C, with the catalytic residues cysteine and lysine replaced by glycine and glutamine, respectively, which are inherently unable to mediate hydrolysis. Furthermore, phylogenetic analysis revealed that CJ1056C and its orthologs from the Campylobacterota phylum form a unique cluster that is evolutionarily separate from catalytically active nitrilase superfamily proteins. Taken together, these findings establish CJ1056C as the first structurally characterized pseudoenzyme of the nitrilase superfamily, presumably lacking conventional nitrilase or amidase activities. Structural analysis of the unique nitrilase superfamily protein CJ1056C from Campylobacter jejuni.,Ahn SY, Yoon SI Biochem Biophys Res Commun. 2025 Sep 20;785:152688. doi: , 10.1016/j.bbrc.2025.152688. PMID:41005285 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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