9xfs

Structure of glutamine amidotransferase DnfC from Alcaligenes sp.

spinqualitylabelsall models 9xfs, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9xfs is a 1 chain structure with sequence from Alcaligenes ammonioxydans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0ABX8SVV5_9BURK Publication Abstract from PubMed Microbial ammonia oxidation is essential for biogeochemical nitrogen cycling and wastewater treatment. Besides the well-studied nitrification and anaerobic ammonia oxidation, a novel ammonia oxidation process referred to as direct ammonia oxidation (dirammox) was recently discovered in heterotrophic nitrifier Alcaligenes members, where ammonia was converted to glutamine and oxidized to hydroxylamine and then to N(2) via a gene cluster, dnfABC. Two possible ammonia oxidation mechanisms were proposed, 1) glutamine is converted to some unknown compounds by potential glutamine amidotransferase DnfC and then oxidized to hydroxylamine by oxidase DnfAB, and 2) glutamine is oxidized to l-glutamic acid gamma-hydroxamate (L-GlngammaHXM) by DnfAB and then hydrolyzed to hydroxylamine by DnfC. Here, we determined the crystal structure of DnfC and identified a conserved catalytic pocket essential for hydroxylamine production and far larger than that required to accommodate a glutamate molecule. We found that the L-GlngammaHXM hydrolysis activity is not necessary for hydroxylamine production in E. coli cells harboring dnfABC. Our structural and functional study of DnfC suggested that glutamine was converted to a so-far unknown compound and sequentially oxidized to hydroxylamine and N(2). Structural and functional study suggests DnfC is a putative glutamine amidotransferase in the dirammox pathway.,Wang XK, Qin YL, Zhao RX, Zhang YB, Guo L, Jiang CY, Qiu JG, Liu SJ, Li DF Biochem Biophys Res Commun. 2026 Jun 4;816:153715. doi: , 10.1016/j.bbrc.2026.153715. Epub 2026 Apr 1. PMID:41936240 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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