9y1s
Rhobin9, de novo rhodamine binder, apo form
Structural highlights
Publication Abstract from PubMedFluorescent imaging in live cells is a cornerstone of life sciences. While natural fluorescent proteins have been engineered to enhance individual features, no existing tag combines ideal properties into a single system: high brightness, reversible binding, compact size, and stability across diverse conditions. Here, we achieve this through de novo design of rhodamine binders (Rhobin). To harness the broad repertoire of rhodamine fluorophores, we developed a generalizable design strategy for a pan-rhodamine binder compatible with diverse wavelengths and applications. Rhobin enables live- and fixed-cell imaging of various subcellular targets in mammalian cells, showing brightness surpassing existing tags. Its reversible fluorophore binding supports super-resolution stimulated emission depletion (STED) and live-cell single-molecule imaging for extended durations compared with HaloTag. Beyond conventional systems, Rhobin enables live imaging of the extremophile Sulfolobus acidocaldarius at 75 degrees C, previously inaccessible with current tags. Together, these results establish Rhobin as a versatile platform for next-generation imaging and biosensor design. De novo pan-rhodamine binders for fluorescence microscopy from mammalian cells to extremophiles.,Chen Y, Yserentant K, Hong K, Chen K, Kuang Y, Picardo RS, Bhowmick A, Charles-Orszag A, Lord SJ, Lu L, Hou K, Mann SI, Bhattacharya S, Horst M, Grimm JB, Lavis LD, Mullins RD, DeGrado WF, Huang B Cell. 2026 Sep 1:S0092-8674(26)00934-7. doi: 10.1016/j.cell.2026.08.007. PMID:42679818[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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