9yzy

ctSAGA Focused on HAT Bromodomain region

spinqualitylabelsall models 9yzy, resolution 4.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9yzy is a 3 chain structure with sequence from Chaetomium thermophilum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.4Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G0SCB2_CHATD Publication Abstract from PubMed The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 A and three of the four HAT subunits at 3.7 A. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex. Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.,Mattoo RUH, Chen DH, Bushnell DA, Tamir S, Kornberg RD Mol Cell. 2025 Nov 18:S1097-2765(25)00866-4. doi: 10.1016/j.molcel.2025.10.025. PMID:41260211 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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