9z8f

Destabilized open state sheep connexin-46 in DMPC nanodiscs at neutral pH

spinqualitylabelsall models 9z8f, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9z8f is a 12 chain structure with sequence from Ovis aries. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.2Å Ligands: MC3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CXA3_SHEEP Publication Abstract from PubMed Gap junctions, formed by connexin proteins, establish direct electrical and metabolic coupling between cells, enabling coordinated tissue responses. These channels universally respond to intracellular pH changes, closing under acidic conditions to limit the spread of cytotoxic signals during cellular stress, such as ischemia. Using cryo-electron microscopy (cryo-EM), we uncover insights into the structural mechanism of pH-gating in native lens connexin-46/50 (Cx46/50) gap junctions. Mild acidification drives lipid infiltration into the channel pore, displacing the N-terminal (NT) domain and stabilizing pore closure. Lipid involvement is shown to be both essential and fully reversible. Structural transitions involve an ensemble of gated states formed through non-cooperative NT domain movement as well as minor populations of a distinct destabilized open-state. These findings provide molecular insights into pH-gating dynamics, illustrating how structural changes may regulate gap junction function under cellular stress and linking Cx46/50 dysregulation to age-related cataract formation. Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM.,Jarodsky JM, Myers JB, Reichow SL Nat Commun. 2026 Jan 12. doi: 10.1038/s41467-026-68311-9. PMID:41526355 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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