Function
Beta-N-acetylhexosaminidase or Beta-hexosaminidase or chitobiase (BNAHEX) is a glycoside hydrolase catalysing the cleavage of GlcNAc and GalNAc [1]. BNAHEX serves as a biosynthetic tool for the preparation of hexosaminides under mild conditions. See also Beta-Hexosaminidase.
Disease
Elevated levels of BNAHEX were found in patients wit Tay-Sachs disease[2].
Relevance
BVAHEX is a target for the design of agricultural fungicides[3].
Structural highlights
The 3D structure of beta-N-acetylhexosaminidase complex with the inhibitor berberine shows the berberine forming π-π stacking interaction interaction with W490. The active site includes a hydrophobic recess interacting with berberine[4]. Whole active site. Water molecules are shown as red spheres.
3D structures of beta-N-acetylhexosaminidase
Beta-N-acetylhexosaminidase 3D structures
- ↑ Slamova K, Bojarova P, Petraskova L, Kren V. beta-N-acetylhexosaminidase: what's in a name...? Biotechnol Adv. 2010 Nov-Dec;28(6):682-93. doi: 10.1016/j.biotechadv.2010.04.004., Epub 2010 May 9. PMID:20438826 doi:https://dx.doi.org/10.1016/j.biotechadv.2010.04.004
- ↑ Bayleran J, Hechtman P, Kolodny E, Kaback M. Tay-Sachs disease with hexosaminidase A: characterization of the defective enzyme in two patients. Am J Hum Genet. 1987 Oct;41(4):532-48. PMID:2959149
- ↑ Horsch M, Mayer C, Sennhauser U, Rast DM. Beta-N-acetylhexosaminidase: a target for the design of antifungal agents. Pharmacol Ther. 1997 Oct-Dec;76(1-3):187-218. doi: 10.1016/s0163-7258(97)00110-1. PMID:9535180 doi:https://dx.doi.org/10.1016/s0163-7258(97)00110-1
- ↑ Duan Y, Liu T, Zhou Y, Dou T, Yang Q. Glycoside hydrolase family 18 and 20 enzymes are novel targets of the traditional medicine berberine. J Biol Chem. 2018 Oct 5;293(40):15429-15438. doi: 10.1074/jbc.RA118.004351. Epub , 2018 Aug 22. PMID:30135205 doi:https://dx.doi.org/10.1074/jbc.RA118.004351
