Guide-independent Pseudouridine synthase

spinqualitylabelsall models tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate 1ze2 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function The alternative to using the RNA-guided mechanism for pseudouridine formation is a guide-independent mechanism acting through stand-alone enzymes. In eukaryotes, these enzymes are called Pus enzymes (Pseudouridine synthases), and they modify uridine by recognizing sequence and/or secondary structural elements of the RNA containing the target uridine. pseudouridine synthase (PUS) catalyzes the conversion of uridine to tRNA pseudouridine in several types of RNA molecules, . tRNAs are well-characterized as targets of these protein-only pseudouridine synthases, but recently have even been found to act on messenger RNAs in a variety of eukaryotic organisms, including humans, , . In eubacteria PUS are active in the ribosomal large subunit Ribosomal large subunit pseudouridine synthase and in the ribosomal small subunit Ribosomal small subunit pseudouridine synthase. Ribosomal large subunit pseudouridine synthases: RluA is responsible for synthesis of pseudouridine from uracil in positions 746 in 23S ribosomal RNA and in the anticodon loop of tRNAPhe, tRNACys and tRNALeu. RluB is responsible for synthesis of pseudouridine from uracil in position 2605 in 23S ribosomal RNA. RluC is responsible for synthesis of pseudouridine from uracil in positions 955, 2504 and 2580 in 23S ribosomal RNA. RluD is responsible for synthesis of pseudouridine from uracil in positions 1911, 1915 and 1917 in 23S ribosomal RNA. RluE is responsible for synthesis of pseudouridine from uracil in position 2457 in 23S ribosomal RNA. RluF is responsible for synthesis of pseudouridine from uracil in position 2604 in 23S ribosomal RNA. Ribosomal small subunit pseudouridine synthase RsuA is responsible for synthesis of pseudouridine from uracil in positions 516 in 16S ribosomal RNA. Structural highlights The conversion of uridine to pseudouridine causes conformational change of PUS. tRNA pseudouridine synthase binding with pseudouridine monophosphate (1ze2).

3D Structures of guide-independent pseudouridine synthase

Updated on 13-November-2018

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• tRNA pseudouridine synthase
  • 1ze2 – EcPUS I – Escherichia coli
    
  • 4iqm, 4its, 4j37, 4nz6 - hPUS Pus1 catalytic domain - human
    
  • 4nz7 - hPUS Pus1 catalytic domain (mutant)
    
  • 1vs3 – PUS TruA – Thermus thermophilus
    
  • 1sgv – PUS TruB – Mycobacterium tuberculosis
    
  • 1si7, 1szw, 1sb7 - EcPUS TruD
• tRNA pseudouridine synthase complex with RNA
  • 1k8w – EcTruB + stem-loop RNA
    
  • 1zl3 - EcTruB (mutant) + stem-loop RNA
    
  • 2nqp, 2nr0, 2nre – EcTruA + Leu-tRNA
    
  • 1ze2 - TmPUS B + RNA
    
  • 2ab4 - TmPUS B (mutant) + RNA
    
  • 1r3e, 1r3f - EcTruB + RNA
    
• Probable tRNA pseudouridine synthase
  • 3hjw, 3lwo, 3lwp, 3lwq, 3lwr, 3lwv, 3hax – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – Pyrococcus furiosus
    
  • 3hay – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein
    
  • 2hvy – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein + small nuclear RNP Gar1-like protein
    
  • 3mqk - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
    
  • 2rfk - PfPUS Cbf5 (mutant) + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
    
  • 2ey4 - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein
    
  • 3hjy - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA
    
  • 2apo - PfPUS Cbf5 + ribosome biogenesis protein NOP10 - Methanocaldococcus jannaschii
    
• Ribosomal large subunit pseudouridine synthase
  • 2i82 - EcRluA catalytic domain + RNA
    
  • 4lab - EcRluB catalytic domain
    
  • 4lgt - EcRluB catalytic domain + RNA
    
  • 1xpi - EcRluC catalytic domain
    
  • 5vbb - hRluC-like
    
  • 1prz, 1qyu, 1v9f, 1v9k, 2ist - EcRluD catalytic domain
    
  • 2oml, 2olw - EcRluE catalytic domain
    
  • 2gml - EcRluF catalytic domain
    
  • 3dh3 - EcRluF catalytic domain + RNA
    
• Ribosomal small subunit pseudouridine synthase
  • 1vio - RsuA - Haemophilus influenzae
    
  • 1ksk - EcRsuA
    
  • 1ksl - EcRsuA + uracil
    
  • 1ksv - EcRsuA + UMP
    

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Wayne Decatur