Function
Matriptase or suppressor of tumorigenicity 14 protein (ST14) is an epithelial-derived, membrane multi-domain serine protease. ST14 cleaves and activates hepatocyte growth factor/scatter factor and urokinase plasminogen. Benzamidine is an inhibitor of ST14. ST14 catalytic domain contains residues 615-855[1].
Relevance
ST14 has a role in ovarian cancer and is a target for anti-cancer therapy[2]. Inflammation-associated reactive oxygen species and tissue acidity enhance ST14 activation in some skin diseases[3].
Structural highlights
ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the Ser-His-Asp catalytic triad and Gly-Ser oxyanion hole. The inhibitor benzamidine blocks the catalytic triad[4]. Water molecules shown as red spheres.
3D Structures of matriptase
Matriptase 3D structures
- ↑ List K, Bugge TH, Szabo R. Matriptase: potent proteolysis on the cell surface. Mol Med. 2006 Jan-Mar;12(1-3):1-7. PMID:16838070 doi:https://dx.doi.org/10.2119/2006-00022.List
- ↑ Johnson MD, Oberst MD, Lin CY, Dickson RB. Possible role of matriptase in the diagnosis of ovarian cancer. Expert Rev Mol Diagn. 2003 May;3(3):331-8. PMID:12779007 doi:https://dx.doi.org/10.1586/14737159.3.3.331
- ↑ Chen CJ, Wu BY, Tsao PI, Chen CY, Wu MH, Chan YL, Lee HS, Johnson MD, Eckert RL, Chen YW, Chou F, Wang JK, Lin CY. Increased matriptase zymogen activation in inflammatory skin disorders. Am J Physiol Cell Physiol. 2011 Mar;300(3):C406-15. doi:, 10.1152/ajpcell.00403.2010. Epub 2010 Dec 1. PMID:21123732 doi:https://dx.doi.org/10.1152/ajpcell.00403.2010
- ↑ Friedrich R, Fuentes-Prior P, Ong E, Coombs G, Hunter M, Oehler R, Pierson D, Gonzalez R, Huber R, Bode W, Madison EL. Catalytic domain structures of MT-SP1/matriptase, a matrix-degrading transmembrane serine proteinase. J Biol Chem. 2002 Jan 18;277(3):2160-8. Epub 2001 Nov 5. PMID:11696548 doi:10.1074/jbc.M109830200
