SANDBOX YEAST TATA-BOX-BINDING PROTEIN/TATA-BOX COMPLEX
Yeast TATA-Box-Binding Protein/TATA-Box Complex
ContentsOverviewThe 2.5 Å crystal structure of a TATA-box complex with yeast TATA-box-binding protein (TBP) is shown at the right (1ytb is a two chain structure with sequence from Saccharomyces cerevisiae that is bound to the TATA-box complex of DNA). [1] A key initial step in TATA-box promoters is the recognition by TBP of the TATA box. [2] TBP initiates strand separation on binding to DNA. [2] As a key factor in the transcription preinitiation complex, TBP helps situate the RNA polymerase II over a gene’s transcription start site. [2] StructureIts characteristic saddle-shape consists of two similar domains. The saddle-shape of TBP provides docking sites that allow other transcription factors and proteins to bind. [2] TBP and numerous TBP-associated factors interact to make TFIID, a transcription factor that is part of the active RNA polymerase II transcription complex. [2] TBP is a 30-kd component of the 700-kd TFIID complex. [2] TBP-DNA interactions contain many important structural features. One important feature of the TBP is that its long dimension follows the trajectory of the minor groove of the DNA. [3] TBP interacts with the DNA through the concave side of the β-sheet. [1] Conserved between the two subdomains are two rows of basic residues that lie along the edges of the protein in proximity to the phosphates in DNA. [3] Down the middle of the groove between the two rows of basic residues lies a band of hydrophobic residues runs down the center of the DNA-binding surface. [3] Mechanism for Binding to DNAThe concave surface of TBP is the site where eight base pairs of the TATA box of DNA binds. [1] On binding, it induces large conformation changes in the bound DNA where it bends the major groove of DNA with unprecedented severity and causes the double helix to become significantly underwound. [1] The purpose of the conformation changes is to widen the double helix’s minor groove, which allows the DNA to render extensive contact with the antiparallel β strands that are located on the concave side of the TBP. [2] The main types of interactions at this interface are hydrophobic interactions between the minor groove and the entire hydrophobic under-surface of the TBP saddle [2] At the binding site, four Phenylalanine residues intercalate themselves between the TATA box base pairs on the DNA. [2] The resulting severe bend and positive writhe alters the trajectory of the flanking B-form DNA. [1] The Lys 133, 145, 151 are involved in the function of recruiting TFIIA. [4] Role as Transcription Factor SubunitTBP acts as a 30-kd subunit of the 700-kd eukaryotic transcription factor TFIID. [2] TBP and a variety of TBP-associated factors, make up the TFIID, which in turn makes up part of the RNA polymerase II preinitiation complex. [2] During the formation of the preinitiation transcription complex of RNA polymerase II, TFIID is the first protein to bind to DNA. [2] The recruitment of other factors required for RNA Polymerase II to initiate transcription results from the binding of TFIID to the TATA box in the promoter region of the gene. [2] The surface of the TBP saddle provides docking sites for the binding of other transcription factors. [2] TFIIA is recruited and interacts primarily with the TBP. [2] TFIIB and TFIIF are recruited and are followed by the RNA polymerase II, TFIIE, and TFIIH. This results in the formation of the basal transcription apparatus. [2]
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