User:Coline Perrin/CCL2

spinqualitylabelsall models Monocyte chemoattractant protein 1 (PDB code 3idf) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function and Structure Human synthetic monocyte chemoattractant protein 1 (MCP) belongs to the superfamily of chemokines, which are proteins involved in immunoregulatory and inflammatory processes. The gene for CCL2 is on chromosome 17 in region 17q11.2-q12. The superfamily can be subdivided into 4 smaller groups, depending on the N-ter arangment of the cysteines. The CCL2 is also known as chemokine (C-C motif) ligand or: - MCP1 - small inducible cytokine A2 (SCYA2) - MCAF - GDCF-2 - SMC-CF - HSMCR30 - MGC9434 - GDCF-2 - HC11. It exists as a monomer or a dimer, eventhough the homodimer form is preferred. The structure of the monomer is made of 3 Beta sheets and 1 alpha helix. Ligands The known ligands for CCL2 are Potassium and PO4. The potassium binds to the S33 and S34 of the monomer and PO4 binds to F15 and N17. Evolutionary Conservation Check, as determined by ConSurfDB. Diseases CCL2 is implicated in several diseases like psoriasis and rheumatoid arthritis where the appear to recruit macrophages, therefore bolstering the inflammation on joints. It is thought to be involved in atherosclerosis in the recruitment of monocytes into the arterial wall as well as in prostate cancer. It has also been found elevated in the urine of people with lupus as a sign warning of inflammation of the kidney. CCL2 is overexpressed in epilepsy, brain ischemia, Alzheimer's disease, EAE and traumatic brain injury. Structural highlights CCL2 is part of the C-C motif group because of the covalent bond made between 2 of the 4 cysteines of the N terminal domain. Post translational modifications at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

Synthesis

The protein human CCL2 has been synthesized using a combination of solid phase peptide synthesis (SPPS) and native chemical ligation (NCL). The thioester-peptide segment was synthesized using the sulfonamide safety-catch linker and 9-fluorenylmethoxycarbonyl (Fmoc) SPPS, and pseudoproline dipeptides were used to facilitate the synthesis of both CCL2 fragments. After assembly of the full-length peptide chain by NCL, a glutathione redox buffer was used to fold and oxidize the CCL2 protein. CCL2 was crystallized and the structure was determined by X-ray diffraction at 1.9-A resolution. This is probably one of the first crystal structures of a protein prepared using the sulfonamide safety-catch linker and NCL.

3D structures of Monocyte chemoattractant protein

Updated on 27-January-2016

{"openlevels":0}

• Monocyte chemoattractant protein 1
  • 1dok, 1dol – hMCP-1 (mutant) - human
    
  • 3ifd – hMCP-1
    
  • 1dom, 1don – hMCP-1 - NMR
    
  • 1ml0, 2nz1 – hMCP-1 (mutant) + M3 protein
    
  • 2bdn – hMCP-1 + antibody
    
  • 4dn4 – hMCP-1 (mutant) + antibody
    
  • 4zk9 – hMCP-1 + chemokine binding protein
    
  • 4r8i – hMCP-1 + RNA
    
• Monocyte chemoattractant protein 2
  • 1esr – hMCP-2 (mutant)
    
• Monocyte chemoattractant protein 3
  • 1ncv, 1bo0 – hMCP-3 - NMR
    
  • 4zkc – hMCP-3 + chemokine binding protein
    
• Monocyte chemoattractant protein 4
  • 2ra4 – hMCP-4
    

References

https://fr.wikipedia.org/wiki/CCL2 https://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1DOK https://www.uniprot.org/uniprot/P13500#interaction https://www.rcsb.org/pdb/explore/explore.do?structureId=3IFD

Proteopedia Page Contributors and Editors (what is this?)

Coline Perrin