User:Tilman Schirmer/Sandbox 201
PleD
Overview
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PleD from Caulobacter crescentus is a response regulator with an unorthodox catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver (Rec) domain,
a Rec-like (Rec') adaptor domain,
and a C-terminal GGDEF domain that confers the catalytic acitvity.
The GGDEF domain is named after the highly conserved signature motif (in PleD it is GGEEF) that locates to a β-hairpin.
Substrate binding
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The motif is part of the substrate binding site as identified in the structure of PleD in complex with GTP-alpha-S / Mg++. The GGDEFY domain binds only one GTP subsrate molecule. For the reaction to proceed, two GTP loaded GGDEF domains have to align antiparallely. MODEL.
Allosteric product binding site
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C-di-GMP
Primary inhibition site (Ip)
Secondary inhibition site (Is)
Primary and secondary inhibition sites
Primary and secondary inhibition sites
Primary and secondary inhibition sites + 5GP
Two conformations
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