Vacuolar protein sorting-associated protein

spinqualitylabelsall models Vps75 (deepskyblue and green) complex with histone acetyltransferase (magenta) and sulfate (PDB code 3q68) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Vacuolar protein sorting-associated protein (Vps) are part of Endosomal Sorting Complex Required for Transport (ESCRT) that performs the topologically unique membrane bending and scission reaction away from the cytoplasm. This process is required for the multivesicular body (MVP) pathway, cytokinesis and HIV budding. There are five distinct ESCRT complexes (0,I,II,III,Vps4) with distinct functions. The retromer comprises 2 functional subcomplexes: the cargo-selective one which contains Vps35, Vps29 and Vps26 and the sorting nexin subcomplex which tubulate the endosomal membrane. The Vps proteins are found in yeast. The human homologs are called also chromatin-modifying protein or Charged multivesicular body protein (CHMP). Vps4, Vps4A, Vps4B complex catalyzes the disassembly of the ESCRT-III filament in an ATP-driven reaction. Vps4 proteins are required for centrosome and spindle maintenance. Vps9A activates Rab5 to GTP-bound form. Vps15 may function as a G protein β subunit at the endosome . Vps23 is involved in appropriate sorting of receptors within the yast endocytic pathway and is part of the ESCRT-I complex Vps25 is part of the ESCRT-II complex which contains two copies of the protein. It induces a conformational switch that converts inactive Vps20 into an active nucleator for Snf7 oligomerization. Vps26A and Vps26B are part of the Vps29-Vps35-Vps26 retromer which mediates transport of transmembrane proteins from endosomes to the trans-Golgi network. Vps27 interacts with the lipid phosphatidylinositol-3-phosphate and this interaction activates the MVP sorting pathway. Vps27 is part of the ESCRT-I complex. Vps28 C-terminal links ESCRT-I and ESCRT-II. Vps29, Vps30 and Vps35 are required for the recycling of Vps10p from the prevacuolar endosome back to the Golgi. Vps33 binds SNARE domains. Vps33A is part of the homotypic fusion and vacuole sorting comples which is required for fusion of intracellular compartments with lysosomes. Vps34 is a class III Phosphoinositide 3-Kinases. Vps36 contains a ubiquitin-binding domain and is required for vacuolar sorting of ubiqinated membrane proteins. Vps36 is part of the ESCRT-II complex. Vps46 is part of the ESCRT-II complex. Vps53 C-terminal is important for binding endosome-derived vesicles. Vps54 is part of the Golgi-associated retrograde protein complex (GARP) of vesicle sorting proteins. Vps60 is involved in filament maturation. Vps74 is a sensor of phosphatidylinositol 4-kinase level on medial Golgi cisternae. Vps75 is a histone chaperone which functions in chromatin assembly and disassembly. Vps Vta1 is a cofactor ov Vps4. Disease Mutations in Vps53 cause progressive cerebello-cerebral atrophy type 2. Mutations in Vps54 in mice cause motor neuron disease. Dysfunction of the retromer is implicated in neurodegenerative disease like Alzheimer disease, Parkinson's disease and Frontotemporal lobar degeneration. Relevance Vps25 in Drosophila possesses several properties of tumor repressor. Vps36 is downregulated in advanced prostate cancer. Vps26A Structural highlights Vps75 interacts with histone acetyltransferase through three interfacing areas. The main interface area contains a globular motif that surrounds Vps75 Leu223 and several hydrogen bonds and salt bridges. The other two interfaces are on each side of the catalytic enclosure and contain hydrophobic contacts and salt bridges. 2nd interface. 3rd interface. 3D structures of vacuolar protein sorting-associated protein Vacuolar protein sorting-associated protein 3D structures

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky