View source for 10zy


==Yeast co-transcriptional pre-60S assembly intermediate Pwp1 RNP==
<StructureSection load='10zy' size='340' side='right'caption='[[10zy]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[10zy]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10ZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10ZY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.74&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10zy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10zy OCA], [https://pdbe.org/10zy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10zy RCSB], [https://www.ebi.ac.uk/pdbsum/10zy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10zy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL8A_YEAST RL8A_YEAST] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Eukaryotic ribosomal large subunit (60 S ) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60 S assembly intermediates. The underlying molecular mechanisms responsible for nucleation of pre-60 S assembly, quality control, and installation of ITS2 during co-transcriptional stages remain unknown. Here we report the earliest co-transcriptional assembly intermediates of the eukaryotic 60 S subunits. Together with biochemical assays, our data reveal the architecture of co-transcriptional pre-60 S assembly initiation and progression, as well as the molecular logic of an assembly checkpoint. This study highlights an evolutionary solution by which complex RNA folding processes can be parallelized and integrated via biological AND-gating.

Mechanism for the initiation of co-transcriptional pre-60 S assembly.,Piwowarczyk R, Klinge S bioRxiv [Preprint]. 2026 May 22:2026.05.22.727207. doi: , 10.64898/2026.05.22.727207. PMID:42239465<ref>PMID:42239465</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 10zy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Klinge S]]
[[Category: Piwowarczyk R]]