View source for 2xbo


==Equine Rhinitis A Virus in Complex with its Sialic Acid Receptor==
<StructureSection load='2xbo' size='340' side='right'caption='[[2xbo]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xbo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_rhinitis_A_virus Equine rhinitis A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XBO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900025:3-sialyl-alpha-lactose'>PRD_900025</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xbo OCA], [https://pdbe.org/2xbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xbo RCSB], [https://www.ebi.ac.uk/pdbsum/2xbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xbo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B9VV85_9PICO B9VV85_9PICO] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/2xbo_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xbo ConSurf].
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== Publication Abstract from PubMed ==
Equine rhinitis A virus (ERAV) shares many features with foot-and-mouth disease virus (FMDV) and both are classified within the genus Aphthovirus of the family Picornaviridae. ERAV is used as a surrogate for FMDV research as it does not require high-level biosecurity. In contrast to FMDV, which uses integrins as cellular receptors, the receptor for ERAV has been reported to involve the sugar moiety sialic acid. This study confirmed the importance of sialic acid for cell entry by ERAV and reports the crystal structure of ERAV particles complexed with the receptor analogue 3'-sialyllactose. The receptor is attached to the rim of a capsid pit adjacent to the major immunogenic site and distinct from the sialic acid binding site used by a related picornavirus, the cardiovirus Theiler's murine encephalitis virus. The structure of the major antigenic determinant of the virus, previously identified from antibody escape mutations, is also described as the EF loop of VP1, which forms a hairpin stretching across the capsid surface close to the icosahedral fivefold axis, neighbouring the receptor-binding site, and spanning two protomeric units.

Crystal structure of equine rhinitis A virus in complex with its sialic acid receptor.,Fry EE, Tuthill TJ, Harlos K, Walter TS, Rowlands DJ, Stuart DI J Gen Virol. 2010 Aug;91(Pt 8):1971-7. Epub 2010 Apr 28. PMID:20427563<ref>PMID:20427563</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Equine rhinitis A virus]]
[[Category: Large Structures]]
[[Category: Fry EE]]
[[Category: Harlos K]]
[[Category: Rowlands DJ]]
[[Category: Stuart DI]]
[[Category: Tuthill TJ]]
[[Category: Walter TS]]