View source for 3iir


==Crystal Structure of Miraculin like protein from seeds of Murraya koenigii==
<StructureSection load='3iir' size='340' side='right'caption='[[3iir]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3iir]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murraya_koenigii Murraya koenigii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IIR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iir OCA], [https://pdbe.org/3iir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iir RCSB], [https://www.ebi.ac.uk/pdbsum/3iir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iir ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D2YW43_MURKO D2YW43_MURKO] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/3iir_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iir ConSurf].
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== Publication Abstract from PubMed ==
Earlier, the purification of a 21.4kDa protein with trypsin inhibitory activity from seeds of Murraya koenigii has been reported. The present study, based on the amino acid sequence deduced from both cDNA and genomic DNA, establishes it to be a miraculin-like protein and provides crystal structure at 2.9A resolution. The mature protein consists of 190 amino acid residues with seven cysteines arranged in three disulfide bridges. The amino acid sequence showed maximum homology and formed a distinct cluster with miraculin-like proteins, a soybean Kunitz super family member, in phylogenetic analyses. The major differences in sequence were observed at primary and secondary specificity sites in the reactive loop when compared to classical Kunitz family members. The crystal structure analysis showed that the protein is made of twelve antiparallel beta-strands, loops connecting beta-strands and two short helices. Despite similar overall fold, it showed significant differences from classical Kunitz trypsin inhibitors.

Cloning, sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii.,Gahloth D, Selvakumar P, Shee C, Kumar P, Sharma AK Arch Biochem Biophys. 2010 Feb 1;494(1):15-22. Epub 2009 Nov 13. PMID:19914199<ref>PMID:19914199</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3iir" style="background-color:#fffaf0;"></div>

==See Also==
*[[Miraculin|Miraculin]]
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Murraya koenigii]]
[[Category: Gahloth D]]
[[Category: Kumar P]]
[[Category: Selvakumar P]]
[[Category: Sharma AK]]
[[Category: Shee C]]