View source for 3ory


==Crystal structure of Flap endonuclease 1 from hyperthermophilic archaeon Desulfurococcus amylolyticus==
<StructureSection load='3ory' size='340' side='right'caption='[[3ory]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ory]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfurococcus_amylolyticus Desulfurococcus amylolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ORY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ORY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ory FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ory OCA], [https://pdbe.org/3ory PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ory RCSB], [https://www.ebi.ac.uk/pdbsum/3ory PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ory ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F2Z289_DESAM F2Z289_DESAM] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.[HAMAP-Rule:MF_00614]

==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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</StructureSection>
[[Category: Desulfurococcus amylolyticus]]
[[Category: Large Structures]]
[[Category: Kawarabayashii Y]]
[[Category: Kubota K]]
[[Category: Mase T]]
[[Category: Miyazono K]]
[[Category: Tanokura M]]