View source for 3wpx


==Structure of PomBc4, a periplasmic fragment of PomB from Vibrio alginolyticus==
<StructureSection load='3wpx' size='340' side='right'caption='[[3wpx]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wpx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WPX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wpx OCA], [https://pdbe.org/3wpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wpx RCSB], [https://www.ebi.ac.uk/pdbsum/3wpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wpx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O06874_VIBAL O06874_VIBAL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The torque of the bacterial flagellum is generated by the rotor-stator interaction coupled with the ion flow through the channel in the stator. Anchoring the stator unit to the peptidoglycan layer with proper orientation around the rotor is believed to be essential for smooth rotation of the flagellar motor. The stator unit of the sodium-driven flagellar motor of Vibrio is composed of PomA and PomB, and is thought to be fixed to the peptidoglycan layer and the T-ring by the C-terminal periplasmic region of PomB. Here, we report the crystal structure of a C-terminal fragment of PomB (PomBC) at 2.0-A resolution, and the structure suggests a conformational change in the N-terminal region of PomBC for anchoring the stator. On the basis of the structure, we designed double-Cys replaced mutants of PomB for in vivo disulfide cross-linking experiments and examined their motility. The motility can be controlled reproducibly by reducing reagent. The results of these experiments suggest that the N-terminal disordered region (121-153) and following the N-terminal two-thirds of alpha1(154-164) in PomBC changes its conformation to form a functional stator around the rotor. The cross-linking did not affect the localization of the stator nor the ion conductivity, suggesting that the conformational change occurs in the final step of the stator assembly around the rotor.

Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor.,Zhu S, Takao M, Li N, Sakuma M, Nishino Y, Homma M, Kojima S, Imada K Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13523-8. doi:, 10.1073/pnas.1324201111. Epub 2014 Sep 2. PMID:25197056<ref>PMID:25197056</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wpx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio alginolyticus]]
[[Category: Homma M]]
[[Category: Imada K]]
[[Category: Kojima S]]
[[Category: Sakuma M]]
[[Category: Takao M]]
[[Category: Zhu S]]