View source for 4alz


==The Yersinia T3SS basal body component YscD reveals a different structural periplasmatic domain organization to known homologue PrgH==
<StructureSection load='4alz' size='340' side='right'caption='[[4alz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4alz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ALZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4alz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alz OCA], [https://pdbe.org/4alz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4alz RCSB], [https://www.ebi.ac.uk/pdbsum/4alz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4alz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YSCD_YEREN YSCD_YEREN] Required for the export process of the Yop proteins.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the first three-dimensional structure of Yersinia enterocolitica and Shigella flexneri injectisomes in situ and the first structural analysis of the Yersinia injectisome. Unexpectedly, basal bodies of injectisomes inside the bacterial cells showed length variations of 20%. The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had similar dimensions and were significantly longer than the isolated structures of related injectisomes. The crystal structure of the inner membrane injectisome component YscD appeared elongated compared to a homologous protein, and molecular dynamics simulations documented its elongation elasticity. The ring-shaped secretin YscC at the outer membrane was stretched by 30-40% in situ, compared to its isolated liposome-embedded conformation. We suggest that elasticity is critical for some two-membrane spanning protein complexes to cope with variations in the intermembrane distance. DOI:http://dx.doi.org/10.7554/eLife.00792.001.

In situ structural analysis of the Yersinia enterocolitica injectisome.,Kudryashev M, Stenta M, Schmelz S, Amstutz M, Wiesand U, Castano-Diez D, Degiacomi MT, Munnich S, Bleck CK, Kowal J, Diepold A, Heinz DW, Dal Peraro M, Cornelis GR, Stahlberg H Elife. 2013 Jul 30;2:e00792. doi: 10.7554/eLife.00792. Print 2013. PMID:23908767<ref>PMID:23908767</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4alz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Yersinia enterocolitica]]
[[Category: Cornelis GR]]
[[Category: Heinz DW]]
[[Category: Muenich S]]
[[Category: Schmelz S]]
[[Category: Stenta M]]
[[Category: Widow U]]
[[Category: Wisand U]]