View source for 4cc8


==Pre-fusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy==
<SX load='4cc8' size='340' side='right' viewer='molstar' caption='[[4cc8]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cc8]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. The January 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''HIV Envelope Glycoprotein''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_1 10.2210/rcsb_pdb/mom_2014_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CC8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cc8 OCA], [https://pdbe.org/4cc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cc8 RCSB], [https://www.ebi.ac.uk/pdbsum/4cc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cc8 ProSAT]</span></td></tr>
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== Publication Abstract from PubMed ==
The activation of trimeric HIV-1 envelope glycoprotein (Env) by its binding to the cell-surface receptor CD4 and co-receptors (CCR5 or CXCR4) represents the first of a series of events that lead to fusion between viral and target-cell membranes. Here, we present the cryo-EM structure, at subnanometer resolution ( approximately 6 A at 0.143 FSC), of the 'closed', prefusion state of trimeric HIV-1 Env complexed to the broadly neutralizing antibody VRC03. We show that three gp41 helices at the core of the trimer serve as an anchor around which the rest of Env is reorganized upon activation to the 'open' quaternary conformation. The architecture of trimeric HIV-1 Env in the prefusion state and in the activated intermediate state resembles the corresponding states of influenza hemagglutinin trimers, thus providing direct evidence for the similarity in entry mechanisms used by HIV-1, influenza and related enveloped viruses.

Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy.,Bartesaghi A, Merk A, Borgnia MJ, Milne JL, Subramaniam S Nat Struct Mol Biol. 2013 Oct 23. doi: 10.1038/nsmb.2711. PMID:24154805<ref>PMID:24154805</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4cc8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Art:HIV Glycoprotein|Art:HIV Glycoprotein]]
*[[Gp120 3D structures|Gp120 3D structures]]
*[[Gp41 3D Structures|Gp41 3D Structures]]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
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[[Category: HIV Envelope Glycoprotein]]
[[Category: Homo sapiens]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Bartesaghi A]]
[[Category: Borgnia MJ]]
[[Category: Merk A]]
[[Category: Milne JLS]]
[[Category: Subramaniam S]]