View source for 4gg4


==Crystal structure of the TAL effector dHax3 bound to specific DNA-RNA hybrid==
<StructureSection load='4gg4' size='340' side='right'caption='[[4gg4]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gg4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._armoraciae Xanthomonas campestris pv. armoraciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GG4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.501&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gg4 OCA], [https://pdbe.org/4gg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gg4 RCSB], [https://www.ebi.ac.uk/pdbsum/4gg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gg4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q3ZD72_XANCA Q3ZD72_XANCA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The transcription activator-like (TAL) effector targets specific host promoter through its central DNA-binding domain, which comprises multiple tandem repeats (TALE repeats). Recent structural analyses revealed that the TALE repeats form a superhelical structure that tracks along the forward strand of the DNA duplex. Here, we demonstrate that TALE repeats specifically recognize a DNA-RNA hybrid where the DNA strand determines the binding specificity. The crystal structure of a designed TALE in complex with the DNA-RNA hybrid was determined at a resolution of 2.5 A. Although TALE repeats are in direct contact with only the DNA strand, the phosphodiester backbone of the RNA strand is inaccessible by macromolecules such as RNases. Consistent with this observation, sequence-specific recognition of an HIV-derived DNA-RNA hybrid by an engineered TALE efficiently blocked RNase H-mediated degradation of the RNA strand. Our study broadens the utility of TALE repeats and suggests potential applications in processes involving DNA replication and retroviral infections.

Specific DNA-RNA hybrid recognition by TAL effectors.,Yin P, Deng D, Yan C, Pan X, Xi JJ, Yan N, Shi Y Cell Rep. 2012 Oct 25;2(4):707-13. doi: 10.1016/j.celrep.2012.09.001. Epub 2012, Sep 27. PMID:23022487<ref>PMID:23022487</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gg4" style="background-color:#fffaf0;"></div>

==See Also==
*[[TAL effector|TAL effector]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xanthomonas campestris pv. armoraciae]]
[[Category: Deng D]]
[[Category: Pan XJ]]
[[Category: Shi YG]]
[[Category: Yan CY]]
[[Category: Yan N]]
[[Category: Yin P]]