View source for 4rwr


==2.1 Angstrom Crystal Structure of Stage II Sporulation Protein D from Bacillus anthracis==
<StructureSection load='4rwr' size='340' side='right'caption='[[4rwr]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rwr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RWR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rwr OCA], [https://pdbe.org/4rwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rwr RCSB], [https://www.ebi.ac.uk/pdbsum/4rwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rwr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A6L7HNW1_BACAN A0A6L7HNW1_BACAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial spores are the most resistant form of life known on the Earth and represent a serious problem for i) bioterrorism attack, ii) horizontal transmission of microbial pathogens in the community and iii) persistence in patients and in a nosocomial environment. SpoIID (Stage II Sporulation Protein D) is a Lytic Transglycosylase (LT) essential for sporulation. The LT-superfamily is a potential drug target because they are active in essential bacterial processes involving the peptidoglycan (PGN), which is unique to bacteria. However, the absence of structural information for the sporulation specific LT enzymes has hindered mechanistic understanding of SpoIID. Here, we report the first crystal structures with and without ligands of the SpoIID family from two community relevant spore-forming pathogens, Bacillus anthracis and Clostridium difficile. The structures allow us to visualize the overall architecture, characterize the substrate recognition model, identify critical residues and provide the structural basis for catalysis by this new family of enzymes.

Crystal structures of the SpoIID lytic transglycosylases essential for bacterial sporulation.,Nocadello S, Minasov G, Shuvalova LS, Dubrovska I, Sabini E, Anderson WF J Biol Chem. 2016 May 18. pii: jbc.M116.729749. PMID:27226615<ref>PMID:27226615</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rwr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus anthracis]]
[[Category: Large Structures]]
[[Category: Anderson WF]]
[[Category: Bagnoli F]]
[[Category: Bottomley M]]
[[Category: Dubrovska I]]
[[Category: Falugi F]]
[[Category: Flores K]]
[[Category: Grandi G]]
[[Category: Minasov G]]
[[Category: Nocadello S]]
[[Category: Shuvalova L]]
[[Category: Wawrzak Z]]