View source for 4tko


==Structure of the periplasmic adaptor protein EmrA==
<StructureSection load='4tko' size='340' side='right'caption='[[4tko]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4tko]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TKO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tko OCA], [https://pdbe.org/4tko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tko RCSB], [https://www.ebi.ac.uk/pdbsum/4tko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tko ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O67159_AQUAE O67159_AQUAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Periplasmic adaptor proteins are key components of bacterial tripartite efflux pumps. The 2.85A resolution structure of an MFS (major facilitator superfamily) pump adaptor, Aquifex aeolicus EmrA, shows linearly arranged alpha-helical coiled-coil, lipoyl, and beta-barrel domains, but lacks the fourth membrane-proximal domain shown in other pumps to interact with the inner membrane transporter. The adaptor alpha-hairpin, which binds outer membrane TolC, is exceptionally long at 127A, and the beta-barrel contains a conserved disordered loop. The structure extends the view of adaptors as flexible, modular components that mediate diverse pump assembly, and suggests that in MFS tripartite pumps a hexamer of adaptors could provide a periplasmic seal.

Structure of the periplasmic adaptor protein from a major facilitator superfamily (MFS) multidrug efflux pump.,Hinchliffe P, Greene NP, Paterson NG, Crow A, Hughes C, Koronakis V FEBS Lett. 2014 Jul 1. pii: S0014-5793(14)00519-5. doi:, 10.1016/j.febslet.2014.06.055. PMID:24996185<ref>PMID:24996185</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4tko" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus VF5]]
[[Category: Large Structures]]
[[Category: Crow A]]
[[Category: Greene NP]]
[[Category: Hinchliffe P]]
[[Category: Hughes C]]
[[Category: Koronakis V]]
[[Category: Paterson NG]]