View source for 4uqg


==A new bio-isosteric base pair based on reversible bonding==
<StructureSection load='4uqg' size='340' side='right'caption='[[4uqg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uqg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UQG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=SAY:[(2R,3S,5R)-3-HYDROXY-5-(3-HYDROXY-4-METHANOYL-PHENYL)OXOLAN-2-YL]METHYL+DIHYDROGEN+PHOSPHATE'>SAY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T0T:(1R)-1-{4-[(E)-2-(2-AMINOPHENYL)ETHENYL]PHENYL}-1,4-ANHYDRO-2-DEOXY-5-O-PHOSPHONATO-D-ERYTHRO-PENTITOL'>T0T</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uqg OCA], [https://pdbe.org/4uqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uqg RCSB], [https://www.ebi.ac.uk/pdbsum/4uqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uqg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E1C9K5_GEOSE E1C9K5_GEOSE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report the development of a new heterobase that is held together through reversible bonding. The so-formed cross-link adds strong stabilization to the DNA duplex. Despite this, the cross-link opens and closes through reversible imine bonding. Moreover, even enzymatic incorporation of the cross-link is possible. The new principle can be used to stabilize DNA duplexes and nanostructures that otherwise require high salt concentrations, which may hinder biological applications.

"Post-It" Type Connected DNA Created with a Reversible Covalent Cross-Link.,Tomas-Gamasa M, Serdjukow S, Su M, Muller M, Carell T Angew Chem Int Ed Engl. 2014 Nov 28. doi: 10.1002/anie.201407854. PMID:25446281<ref>PMID:25446281</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uqg" style="background-color:#fffaf0;"></div>

==See Also==
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Carell T]]
[[Category: Mueller M]]
[[Category: Serdjukov S]]
[[Category: Su M]]
[[Category: Tomas-Gamasa M]]